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Abstracts - Pig health

African swine fever virus vaccine strain Asfv-G-∆I177l reverts to virulence and negatively affects reproductive performance

ASFV-G-ΔI177L is not genetically stable and, therefore not safe for use in ASF vaccines. ASF vaccine candidates should be tested for safety in pregnant animals.

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Impact of a phytogenic feed additive on diarrhea incidence, intestinal histomorphology and fecal excretion of F4-fimbriated enterotoxigenic Escherichia coli in post-weaning piglets

Phytogenic feed additive supplementation to piglets challenged with F4-fimbriated enterotoxigenic Escherichia coli reduced the prevalence of fecal E. coli excretion and improved jejunal histomorphology.

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The genetic basis of swine inflammation and necrosis syndrome and its genetic association with post-weaning skin damage and production traits

Piglets genetically less likely to present swine inflammation and necrosis syndrome signs are also more unlikely to suffer combined skin damage after weaning.

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Quantitative risk assessment model of the presence of PED and ASF viruses in spray-dried porcine plasma

Under the current conditions, the model estimated that an inactivation level (log-kill) of 7.0, 7.2, and 7.3 log must be achieved to manufacture a 10-, 15-, and 20-tons batch size, respectively.

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Comparison of a novel rapid sampling method to serum and tonsil scraping to detect PRRSV in acutely infected sows

Results suggest great potential of tonsil-oral scraping as a novel, practical, and rapid tool for PRRSV RNA detection in sows to assess sow herd status.

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A novel virus-like particles vaccine induces broad immune protective against deltacoronavirus in piglets

Virus-like particles are a potential clinical candidate for porcine deltacoronavirus vaccination.

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Pluck-pools as diagnostic samples for PRRSV and PCV2 in abortion material and stillbirths

Pluck-pools can be valuable diagnostic material.

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Blood transcriptomic differences in the immune response under stressful environmental according to stocking density

The study confirmed the biological mechanisms through immunological expression patterns according to stocking density.

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Assessment of temperature and time on the survivability of PRRSV and PEDV on experimentally contaminated surfaces

Time and temperature combinations effectively decrease the concentration of PRRSV and PEDV on different surfaces.

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Mitigation of aerosol and microbial concentration in a weaning piggery by spraying nanobubble ozone water with an ultrasonic sprayer

The average aerosol mass concentration in the test area was over 50% lower than in the control area.

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Can environmental nebulization of lavender essential oil improve welfare and modulate nasal microbiota of growing pigs?

The study suggests lavender essential oil may improve the welfare of growing pigs. Inhalation of lavendar reduced the nasal microbiome diversity.

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African swine fever virus DNA is present in non-biting flies collected from outbreak farms in Romania

This study brings compelling evidence of the presence of the most common synanthropic flies near domestic pig farms carrying ASFV DNA.

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Maternal flavour conditioning combined with organic and inorganic iron-supplemented creep feed on piglet and the effect on performance and haemoglobin status

Maternal flavor conditioning may improve the acceptance and efficacy of iron-supplemented creep feed as an alternative to iron injections in piglets.

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Intradermal needle-free injection prevents ASF transmission, while intramuscular needle injection does not

This study confirmed the hematogenous transmission of African Swine Fever virus between pigs through needle-sharing.

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A diagnostic approach to confirm Mycoplasma hyopneumoniae “Day zero” for pathogen eradication

Sampling guidelines proposed in this study can be used for verification of M. hyopneumoniae infection of gilts following exposure to determine Day 0 of herd closure.

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Gut microbiota affects the estrus return of sows by regulating the metabolism of sex steroid hormones

Changes in gut microbial composition significantly altered the functional capacity of steroid hormone biosynthesis in the gut microbiome.

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Infection prediction in swine populations with machine learning

Models provide daily infection probabilities that can be used as a benchmark to more timely support preventive and control strategies on farms.

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First detection of PCV4 in swine in the United States

This study provides insights into the frequency of detection, tissue distribution, and genetic characteristics of PCV4 in the US.

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Reclassification of ASFV into 7 biotypes using unsupervised machine learning

A new classification of ASFV based on comparisons performed considering the entire encoded proteome.

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A cross-sectional assessment of PRRSV nucleic acid detection by RT-qPCR in serum, ear-vein blood swabs, nasal swabs, and oral swabs

Ear-vein blood swabs, nasal swabs, and oral swabs can be used as surveillance samples for detecting PRRSV RNA by RT-qPCR in weaning-age pigs.

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Space–time dynamics of African swine fever spread in the Philippines

The results here will help inform decisions intended to mitigate the impact of ASF in the Philippines and will contribute to the understanding of the epidemiological dynamics.

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Pig manure treatment strategies for mitigating the spread of antibiotic resistance

The composting process can be an efficient strategy for mitigating the spread of antibiotic resistance in the environment.

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A novel recombinant S-based subunit vaccine induces protective immunity against porcine deltacoronavirus challenge in piglets

Data demonstrate for the first time the protective efficacy of spike-based subunit vaccine, which could be a candidate vaccine against PDCoV.

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333 Emerging Voices: Evaluating the nonspecific protection of an immunostimulant based on heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs

Master's thesis from the Institute for Game and Wildlife Research (IREC), University of Castilla-La Mancha (UCLM). Directed by Prof. Dr. Christian Gortázar Schmidt and Prof. Dr. María de los Ángeles Risalde Moya.

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