Abstracts - Pig health

The U.S. PEDV prototype and the S-INDEL-variant strains induced similar viremia levels in inoculated pigs.

This system may be useful for monitoring sentinel herds in real time, considerably reducing the financial and logistical costs of periodic sampling and increasing the chances of early detection of infection.

We describe here the construction of a novel vaccine strain that confers excellent protection against B. suis in a mouse model of infection.

Results demonstrated remarkable IAV diversity, and the complex, rapid and dynamic evolution of IAV during infection of vaccinated pigs that can only be appreciated with repeated sampling of individual animals and deep sequence analysis.

Our data indicates that although high titers of vaccine-derived MDA reduced homologous virus infection, transmission, and disease, MDA alone was sufficient to induce vaccine associated enhanced respiratory disease upon heterologous infection.

The attenuated S. Typhimurium BBS 866 strain, containing mutations in rfaH and multiple sRNAs, addresses the limitations of current Salmonella vaccines by providing cross-protection against Salmonella serovars in swine without interfering with established monitoring programs for Salmonella surveillance.

The IR method ascertains whether piglets are receiving adequate maternal antibodies until their own immune systems are developed.

These results showed that the highest assurance of providing PRRSV-negative semen to sow farms should be based on rRT-PCR testing of serum collected at the time of semen collection.

The indirect ELISA that we developed with rctCap, the recombinant capsid fragment containing the 217–342 nt repeat domain, was sensitive, specific, and suitable for the large-scale detection of P1 infections in swine.

The present study shows that treatment of several animals in a stable with ceftiofur influences the resistance pattern of intestinal Escherichia coli of the treated as well as untreated animals housed in the same stable.

Our results indicate that during outbreaks of IAV in swine, aerosols and surfaces in barns contain significant levels of IAV potentially representing an exposure hazard to both swine and people.

Under study conditions, a high-pressure wash with cold water plus application of an accelerated hydrogen peroxide or a combined glutaraldehyde and quaternary ammonium disinfectant is effective at inactivating PRRSV.

Improving farmers’ clinical diagnosis ability does not appear sufficient to effectively reduce this risk.

Taken together, the results confirm the widespread presence of PPIV1 in the United States swine herd.

To our knowledge, this is the first report on the identification, prevalence, complete genome sequencing and molecular characterizations of PDCoV in diarrhoeal samples in pigs in China.

The findings will be of utility for a better understanding of ASFV pathogenesis and for the rational designing of safe and efficient vaccines against this virus.

There were similar changes in the GI microbiome associated with carriage of S. enterica regardless of whether the pigs were experimentally challenged with S. enterica or acquired it naturally.

These findings indicate that vaccination with an attenuated S. Typhimurium strain reduces S. enterica shedding, but the reduction is not consistent and the vaccine strain may persist in tissues.

This is the first study to report the Senecavirus A infection in clinically affected pigs outside of North America. Senecavirus A was considered a novel emerging pathogen associated with an important vesicular disease in Brazil.

Due to the difference in immune programming during early life, the systems respond differently to the post-weaning newly acquired microbiota. As a consequence, the gut systems of the treatment groups develop into different homeostasis.

During a routine surveillance project on antimicrobial resistance in commensal Escherichia coli from food animals in China, a major increase of colistin resistance was observed.

To our knowledge, this is the first report demonstrating the absence of a response to vaccination in CSFV persistently infected pigs.

This study assessed the feasibility of detecting antibodies against ASFV in faeces for later implementation in surveillance and control programmes.

The study shows that CRISPR-Cas9 multiplexability can be as high as 62 and demonstrates the possibility that PERVs can be inactivated for clinical application to porcine-to-human xenotransplantation.