Concentration, size distribution, and infectivity of airborne particles carrying swine viruses

When pathogens become airborne, they travel associated with particles of different size and composition. Particle size determines the distance across which pathogens can be transported, as well as the site of deposition and the survivability of the pathogen. Despite the importance of this information, the size distribution of particles bearing viruses emitted by infectious animals remains unknown.

In this study we characterized the concentration and size distribution of inhalable particles that transport influenza A virus (IAV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine epidemic diarrhea virus (PEDV) generated by acutely infected pigs and assessed virus viability for each particle size range. Aerosols from experimentally infected pigs were sampled for 24 days using an Andersen cascade impactor able to separate particles by size (ranging from 0.4 to 10 micrometer (μm) in diameter). Air samples collected for the first 9, 20 and the last 3 days of the study were analyzed for IAV, PRRSV and PEDV, respectively, using quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantified as geometric mean copies/m3 within each size range. IAV was detected in all particle size ranges in quantities ranging from 5.5x10² (in particles ranging from 1.1 to 2.1μm) to 4.3x10⁵ RNA copies/m³ in the largest particles (9.0–10.0μm). PRRSV was detected in all size ranges except particles between 0.7 and 2.1μm in quantities ranging from 6x10² (0.4–0.7μm) to 5.1x104 RNA copies/m³ (9.0–10.0μm). PEDV, an enteric virus, was detected in all particle sizes and in higher quantities than IAV and PRRSV (p < 0.0001) ranging from 1.3x10⁶ (0.4–0.7μm) to 3.5x10⁸ RNA copies/m³ (9.0–10.0μm). Infectious status was demonstrated for the 3 viruses, and in the case of IAV and PRRSV, viruses were isolated from particles larger than 2.1μm.

In summary, our results indicated that airborne PEDV, IAV and PRRSV can be found in a wide range of particle sizes. However, virus viability is particle size dependent.

Carmen Alonso, Peter C. Raynor, Peter R. Davies, Montserrat Torremorell. Concentration, size distribution, and infectivity of airborne particles carrying swine viruses. PLoS ONE 10(8): e0135675. DOI: 10.1371/journal.pone.0135675