X
XLinkedinWhatsAppTelegramTelegram
0
Read this article in:

Development of a highly effective African swine fever virus vaccine against the current epidemic Eurasia strain

Here we report the discovery that deletion of a previously uncharacterized gene, I177L, from the highly virulent ASFV-G produces complete virus attenuation in swine.

6 February 2020
X
XLinkedinWhatsAppTelegramTelegram
0

In the absence of any available vaccines, African Swine Fever (ASF) outbreak containment relies on control and culling of infected animals. Limited cross protection studies suggest that in order to ensure a vaccine is effective it must be derived from the current outbreak strain or at the very least from an isolate with the same genotype. Here we report the discovery that deletion of a previously uncharacterized gene, I177L, from the highly virulent ASFV-G produces complete virus attenuation in swine. Animals inoculated intramuscularly with the virus lacking the I177L gene, ASFV-G-ΔI177L, in a dose range of 102 to 106 HAD50 remained clinically normal during the 28 day observational period. All ASFV-G-ΔI177L-infected animals had low viremia titers, showed no virus shedding, developed a strong virus-specific antibody response and, importantly, they were protected when challenged with the virulent parental strain ASFV-G. ASFV-G-ΔI177L is one of the few experimental vaccine candidate virus strains reported to be able to induce protection against the ASFV Georgia isolate, and the first vaccine capable of inducing sterile immunity against the current ASFV strain responsible for recent outbreaks.

Manuel V. Borca, Elizabeth Ramirez-Medina, Ediane Silva, Elizabeth Vuono, Ayushi Rai, Sarah Pruitt, Lauren G. Holinka, Lauro Velazquez-Salinas, James Zhu, Douglas P. Gladue. Development of a highly effective African swine fever virus vaccine by deletion of the I177L gene results in sterile immunity against the current epidemic Eurasia strain. Journal of Virology Jan 2020, JVI.02017-19; DOI: 10.1128/JVI.02017-19

Article Comments

This area is not intended to be a place to consult authors about their articles, but rather a place for open discussion among pig333.com users.
Leave a new Comment

Access restricted to 333 users. In order to post a comment you must be logged in.

You are not subscribed to this list pig333.com in 3 minutes

Weekly newsletter with all the pig333.com updates

Log in and sign up on the list

Related articles

Related products in the shop

The shop specialized in the pig sector
Advice and technical service
More than 120 brands and manufacturers
You are not subscribed to this list Swine News

Swine industry news in your email

Log in and sign up on the list