Effect of short-term benzoic acid and chlortetracycline treatment of feed on splanchnic metabolism of valine in growing pigs

Microbial deamination does not affect valine availability in growing pigs.

The present study aimed to investigate the effects of short-term feed treatment with benzoic acid (BA) and chlortetracycline (CTC) on portal drained visceral (PDV) metabolism of arterial valine (Val) as well as portal absorption and hepatic uptake of Val in growing pigs fed a low protein diet. Eight female pigs (70 ± 2 kg BW) fitted with permanent indwelling catheters in the abdominal aorta, v. cava, hepatic portal vein, hepatic vein, and the mesenteric vein were used in the study. Pigs were fed a diet based on wheat (72%), soybean meal (12%), and barley (10%), supplemented with crystalline Lys, Met, Thr, Trp, and Val (0.08%), and containing 14.7% crude protein (as fed). Intake was restricted to 3.6% of BW/d. Pigs were randomly assigned to either control (CON; basal diet) or treatment (TRT; basal diet + 10 g BA and 0.7 g CTC/kg feed). Feed was offered in 3 equal sized meals at 8-h intervals and treatments were applied for 24 h. Blood samples were collected hourly during the last 8 h of the treatment period. Primed continuous infusion of L-Val (0.085 ± 0.005 mmol/h) into the v. cava was initiated 1 h before first sampling. Plasma samples were analyzed for AA by GC/MS and C-13 abundance in Val was determined using GC/C/IRMS. Data were analyzed using the MIXED procedure in SAS by a model including the fixed effects of treatment and block and with pig by block designated as a random effect.
The arterial concentration of Val tended (P = 0.07) to increase with TRT (0.47 ± 0.02 mmol/L) compared with CON (0.41 ± 0.02 mmol/L). The PDV extraction of arterial Val (4.6 ± 0.4%) did not differ (P = 0.21) among treatments. The net portal flux of Val tended (P= 0.10) to be less for TRT, but the portal absorption of Val corrected for PDV uptake of arterial Val did not differ (P = 0.82) among treatments. The net hepatic and net splanchnic fluxes of Val were not affected by treatment (P = 0.61 to P = 0.94).

Data indicate that upper gut microbial deamination is without quantitative importance to availability of Val in growing pigs fed a low-protein diet added crystalline Val.

NB Kristensen, RG Engberg, BB Jensen, JV Nørgaard, HD Pulsen, HD Zacho, and NM Sloth, 2010. Journal of Animal Science, 88 (E-Suppl. 2):387.

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Effect of short-term benzoic acid and chlortetracycline treatment of feed on splanchnic metabolism of valine in growing pigs

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