X
XLinkedinWhatsAppTelegramTelegram
0

Resistance mechanisms and farm-level distribution of fecal Escherichia coli isolates resistant to extended-spectrum cephalosporins in pigs in Spain

The aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level.
12 January 2011
X
XLinkedinWhatsAppTelegramTelegram
0
Fecal Escherichia coli isolates showing a phenotype of reduced susceptibility or resistance to extended-spectrum cephalosporins are common among pigs in Spain. The aim of this study was to describe the main beta-lactam resistance mechanisms carried by these strains and their distribution at farm-level.

Twenty-nine E. coli isolates showing reduced susceptibility or resistance to extended-spectrum cephalosporins were collected from a sampling frame of 80 pig farms distributed over 13 Spanish provinces. The survey was carried out at the slaughterhouse level in 2004.
Results

Of the 29 isolates, 21 (72%) met the criteria for a positive phenotypic confirmatory test for extended-spectrum beta-lactamases (ESBL). The following ESBLs were detected: SHV-12 (12 isolates, 41%), CTX-M-1 (three isolates, 10%), CTX-M-9 (three isolates, 10%), and CTX-M-14 (three isolates, 10%). The remaining eight isolates (28%) were phenotypically non-ESBL, with seven of them (24%) showing mutations on the chromosomal ampC gene promoter at positions −42 (C → T), −18 (G → A), −1 (C → T), and 58 (C → T). A multiplex PCR for detection of plasmidic class C beta-lactamases was negative for all isolates.


Different ESBLs and other mechanisms linked to extended-spectrum cephalosporin resistance are widely distributed among fecal E. coli from slaughter pigs in Spain.

E. Escudero, L. Vinué, T. Teshager, C. Torres and M.A. Moreno. Resistance mechanisms and farm-level distribution of fecal Escherichia coli isolates resistant to extended-spectrum cephalosporins in pigs in Spain. Research in Veterinary Science. 2010. Vol. 88 (1): 83-87.

Article Comments

This area is not intended to be a place to consult authors about their articles, but rather a place for open discussion among pig333.com users.
Leave a new Comment

Access restricted to 333 users. In order to post a comment you must be logged in.

You are not subscribed to this list pig333.com in 3 minutes

Weekly newsletter with all the pig333.com updates

Log in and sign up on the list

Related articles

You are not subscribed to this list Swine News

Swine industry news in your email

Log in and sign up on the list