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Each of the four pairs of oligonucleotide primers exclusively amplified the targeted fragment of the specific viruses. The sensitivity of the multiplex PCR using purified plasmid constructs containing the specific viral target fragments was 2.58 × 107, 2.64 × 105, 2.66 × 107 and 2.73 × 105 copies for PRRSV, PCV-2, CSFV and PPV, respectively.
Using the multiplex PCR, co-infections with these four viruses were identified in 26/76 (34.2%) piglets born from sows with reproductive failure in China. This method is a rapid, sensitive and cost-effective diagnostic tool for the routine surveillance of viral infections in pigs.
Y. Jiang, H. Shang, H. Xu, L. Zhu, W. Chen, L. Zhao and L. Fang.Simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction.The Veterinary Journal. 2010. Vol. 183 (2): 172-175.
